Scans have confirmed Liverpool midfielder has Thiago Alcantara suffered a hamstring strain, the PA news agency understands.
The Spain international will now face a spell on the sidelines but the club are hoping the problem is not serious and he could return to action within weeks.
The 31-year-old was forced off early in the second half of the Reds’ opening Premier League match of the new season at Fulham last Saturday.
His absence will be a blow for manager Jurgen Klopp, with Curtis Jones, Diogo Jota and Alex Oxlade-Chamberlain already on the injury list.
Liverpool host Crystal Palace in their second Premier League game next Monday before travelling to Manchester United the following week.
The Reds drew 2-2 at Craven Cottage in their opener after twice coming from behind.