Adam Lallana is being assessed after withdrawing early from his first training session back with the England squad, Press Association Sport understands.
The 30-year-old started Tuesday’s session at St George’s Park with the rest of the 22-man squad but left early to have an unspecified issue evaluated.
The Liverpool midfielder worked with the Three Lions throughout their preparation camp and is looking to add to his 34 caps in the upcoming UEFA Nations League clash with Spain and friendly against Leicester.
Southgate’s squad is already down to 22 men after Raheem Sterling withdrew on Monday evening with a back problem.
Those involved in Premier League action on Sunday left the session early, including rejuvenated Manchester United left-back Luke Shaw.