The DUP has blocked the election of a Speaker at the Stormont Assembly in protest at the Northern Ireland Protocol, sparking a new political crisis in the region.
The party’s leader, Sir Jeffrey Donaldson, said he is sending a “clear message” to the EU and the UK Government about resolving issues with the post-Brexit trading arrangement.
But Sinn Fein vice president Michelle O’Neill accused the DUP of “denying democracy”.
The failure to elect a Speaker leaves the Stormont Assembly unable to function.
The first order of business was for MLAs to sign the roll of membership before an attempt was made to elect a Speaker. Two candidates, Mike Nesbitt of the UUP, and Patsy McGlone, of the SDLP, were nominated but did not receive the necessary support.
The DUP is also refusing to nominate for the position of deputy first minister, which will prevent the forming of a new executive, as part of its protest against the protocol.
Unionists oppose the post-Brexit treaty because of the economic barriers it creates between Northern Ireland and the rest of the UK.
“I believe that we need to send a very clear message to the European Union and to our Government that we are serious about getting this protocol sorted out.
“Because of the harm it is doing, undermining political stability, damaging the agreements that have formed the basis of political progress made in Northern Ireland, to our economy, contributing to the cost-of-living crisis, this matter needs to be dealt with.
“While others sit on their hands we are not prepared to do that.
“We need decisive action taken by the Government.”
He added: “The choice is clear: if the European Union is serious about protecting the political institutions and the Belfast agreement, and its successor agreements, then they know what to do.
“Equally the same message is there for our own Government as well.
“The ball is firmly at the foot of the Government.”
It is the job of politicians to “properly fund” the healthcare service and to agree a three-year budget and invest in the health service, Ms O’Neill said.
“This is our hour of decision, not tomorrow, and not for a moment longer can the DUP deny democracy, punish the public, boycott this Assembly and executive, and prevent us from putting money in people’s pockets.
“Every one party in this chamber told the electorate that they would turn up on day one. Well, the DUP have failed on day one.”
Alliance Party leader Naomi Long warned Sir Jeffrey not to “overplay his hand” in negotiations with the UK Government over the protocol.
“When you play with fire, you will get burned.
“We saw this with Brexit where the DUP had influence, they overplayed their hand and they ended up with the mess that we are now in and they’re now asking us all to fix it,” she said.
“They are playing with fire again, because the institutions in Northern Ireland cannot survive in the way they are being abused.
“The people who need these institutions most and who need stability in Northern Ireland are unionists, so I would caution Sir Jeffrey about assuming that, in another election, there will be willing partners to go into government beyond it or indeed willing people to fight a further election.
“We have just had an election.
“That’s our job as politicians.
“I think the DUP is playing a very dangerous game with the institutions and with the future of Northern Ireland.”
Sir Jeffrey was not in the chamber for the first Assembly meeting as he has chosen to retain his position as an MP, despite being elected as an MLA for Lagan Valley a week ago.
Instead, former party MP Emma Little-Pengelly has been co-opted to replace her leader on the Stormont benches.
As the largest party, the 27 Sinn Fein MLAs took their position on the benches on the right-hand side of the speaker’s chair for the first time.
It is entitled to nominate its Stormont leader Michelle O’Neill as first minister, but she will not be able to take up the role without the DUP nominating a deputy first minister.
Under the rules of the devolved power-sharing administration, both roles are equal and one cannot be in office without the other.
Since last week’s election, Ms O’Neill has repeatedly called for the DUP to re-enter the executive so it can begin to tackle challenges such as the cost-of-living crisis and spiralling hospital waiting lists.
The Stormont sitting comes amid increased tensions between the Westminster Government and the EU over the working of the protocol, which forms part of the UK’s Withdrawal Agreement with the EU.
Foreign Secretary Liz Truss said the UK will have “no choice but to act” if the EU does not show enough “flexibility” over post-Brexit checks on goods going from Britain to Northern Ireland.